Table 1. Deduced Functions of ORFs in the ltm Gene Cluster in Comparison with Those in the mgs Gene Cluster.
| gene | deduced product/amino acida | protein homologuesa,b | % identity/ % similarity | proposed functionc | |
|---|---|---|---|---|---|
| orf(−15)–orf(−1) | beyond the upstream boundary | ||||
| – | MgsA/1217 (ACY01386) | – | transcriptional activatord | ||
| ltmB | LtmB/539 (ACY01397) | MgsB/539 (ACY01387) | 63/71 | type II thioesterase | |
| ltmC | LtmC/88 (ACY01398) | MgsC/83 (ACY01388) | 68/85 | loading module | ACP |
| ltmD | LtmD/656 (ACY01399) | MgsD/656 (ACY01389) | 82/87 | loading module | amidotransferase |
| ltmE | LtmE/3437 (ACY01400) | MgsE/3192 (ACY01390) | 68/74 | module-2, -3, -4 | KS-DH-KR-ACP-KS-B-ACP-KS |
| ltmF | LtmF/8360 (ACY01401) | MgsF/8021 (ACY01391) | 67/73 | module-4, -5, -6, -7, -8, -9, -10 | KR-ACP-KS-ACP-KS-DH-KR-MT-ACP-KS-KR -MT-ACP-KS-KS-DH-KR-ACP-ACP-KS-DH |
| ltmG | LtmG/2098 (ACY01402) | MgsG/1953 (ACY01392) | 58/68 | module-10, -11 | ACP-KS-DH-KR-ACP-TE |
| ltmH | LtmH/768 (ACY01403) | MgsH/751 (ACY01393) | 65/76 | acyltransferase | |
| – | MgsI/338 (ACY01394) | – | oxidoreductased | ||
| – | MgsJ/281 (ACY01395) | – | O-methyltransferased | ||
| ltmK | LtmK/413 (ACY01404) | MgsK/403 (ACY01396) | 32/47 | cytochrome P450 desaturase | |
| ltmL | LtmL/247 (ACY0140%) | Ppta/246 (AAG43513) | 57/65 | phosphopantetheinyltransferase | |
| orf10–orf26 | beyond the downstream boundary | ||||
a
Numbers are in amino acids, and given in parentheses are NCBI accession numbers.
b
Homologues from mgs clusters are given, and other homologues are included if mgs homologues are absent.
c
Domain abbreviations: ACP, acyl carrier protein; B, branching; DH, dehydratase; KR, ketoreductase; KS, ketosynthase; MT, methyltransferase; TE, thioesterase.
d
Function proposed for the genes from the mgs cluster that are missing from the ltm cluster.