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Phosphorylation of the motor domain of hMyo3A by hMyo3A-KD variants: (lane 1) hMyo3A-MDIQo and KD(WT); (lane 2) hMyo3A-MDIQo and KD(T184A); (lane 3) hMyo3A-MDIQo and KD(T188A). The bottom panel indicates input amount of hMyo3A-MDIQo and hMyo3A variants stained with Coomassie Brilliant Blue R-250. The reaction was done with 36 nM of hMyo3A-KD variants, 36 nM of hMyo3A-MDIQo, and 0.25 mM [γ-32P] ATP at 25 °C for 1 h.
