Table 1. Metrics To Determine the Configuration of an Adsorbed Protein Based on Its Labeling Profilea.
| Iads/Isoln | profile = log[Iads/Isoln] | solvent exposure of residues | physical meaning |
|---|---|---|---|
| ≥5.0 | ≥0.70 | more than the native state | accessibility increased by tertiary unfolding |
| 1.25–5.0 | 0.10 to 0.70 | ||
| 0.75–1.25 | –0.12 to 0.10 | similar to the native state | native structure |
| 0.20–0.75 | –0.12 to–0.70 | less than the native state | accessibility decreased by surface or protein–protein effects |
| ≤0.20 | ≤−0.70 |
a
Iads/Isoln values between 0.75 and 1.25 are considered not to be significantly different than that of the native solution-state structure. Iads/Isoln values that are 5× higher or lower than the native solution-state condition are designated as undergoing a high level of change.