Skip to main content
. 2014 Nov 12;86(24):12308–12314. doi: 10.1021/ac5035924

Figure 5.

Figure 5

Absolute quantification of TFRC mRNA copies in single cells. (A) Comparison of single-cell TFRC copy number distributions using FISH or the SD chip. Individual single-cell measurements are presented (left) as well as the distribution of mRNA values (right). The dashed line represents the average copy number for each detection method. The histogram bin size is 150 mRNA copies. The average copy numbers, standard deviations, and distributions were similar for the two methods. (B) False-color mRNA FISH image. Nuclei are colored blue, and TFRC mRNA appear as white spots. The scale bar is 10 μm.