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A timeline of the reprogramming process with four serial transfections of the pOSKM plasmid using G4Arg nanoparticles, from day 0 to day 30.
Abbreviations: D, day; ESC, embryonic stem cell; G4Arg, arginine-terminated generation 4 polyamidoamine; pOSKM, plasmid construct carrying OSKM; pOSKMG, green fluorescent protein (GFP) gene–incorporated pOSKM.
