Table 1.
Flavonoid screen identified inhibitors of APP-Gal4 dependent-luciferase expression
| Flavonoid group | Compound name | Activity compared to control in % 100 nM |
|---|---|---|
| Flavonol | Fisetina | 67.7 ± 11.7 |
| Kaempferol | 103 ± 54.1 | |
| Kaempferol 3O rutinoside | 83.0 ± 44.7 | |
| Quercetin | 98.6 ± 18.1 | |
| Flavone | Apigenin | 90.9 ± 27.9 |
| Apigenin 7O glucoside | 102 ± 13.6 | |
| Coumarin | 137 ± 15.1 | |
| Diosmetin | 90.4 ± 14.3 | |
| Hyperoside | 94.1 ± 14.3 | |
| Sinensetinb | 51.1 ± 26.1* | |
| Flavanone | Hesperetin | 134 ± 14.5 |
| Narirutin | 126 ± 20.7 | |
| Anthocyanin | Cyanidin chloride | 111 ± 39.8 |
| Delphinidin chloride | 114 ± 32.9 | |
| Pelargonidin chloridea | 61.4 ± 6.23 | |
| Flavanol | (+) Catechin | 97.6 ± 14.7 |
| (−)-Epicatechin | 107 ± 35.1 | |
| Epicatechin gallate | 115 ± 28.1 | |
| Epigallocatechinb | 57.2 ± 23.3* | |
| Epigallocatechin gallate | 91.9 ± 33.6 |
Five DIV primary cortical neurons were cotransfected with 0.5 μg pRC-APP-Gal4, pFR-luciferase, and pRL-TK-Renilla, after 0.5 hours; cells were treated with 100 nM flavonoid compound for 24 hours. Cells were lysed and levels of luminescence quantified using the Dual-Glo luciferase kit according to the manufacturer's instructions.
Key: ANOVA, analysis of variance; SD, standard deviation.
a
Flavonoids showing largest inhibition of luciferase expression.
b
Flavonoids showing largest inhibition of luciferase expression and caused significant reduction in luciferase expression (* p = 0.05, 1-way ANOVA with Bonferroni posttest). Values expressed as mean ± SD (n = 4).