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. 2014 Dec 18;10(12):e1004856. doi: 10.1371/journal.pgen.1004856

Figure 2. stk mutant seeds present defects in seed coat PA accumulation.

Figure 2

(A) Sections of wild-type seeds stained with the toluidine blue O revealed the presence of phenolic compounds in the endothelium (ii1). (B) Scheme of Arabidopsis seed coat anatomy. (C) In the stk mutant phenolic compounds are accumulated in the endothelium (ii1) and also in the second layer of the inner integument (ii2, asterisk). (D) Whole-mount vanillin staining confirmed the presence of PAs in the wild-type and (E) in the stk mutant endothelium. In the stk mutant PAs are also accumulated outside the endothelium in the second layer of the inner integument (asterisk). mi, micropyle; en, endothelium. Scale bars = 30 µm (A–E).