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. 2014 Dec 18;10(12):e1004856. doi: 10.1371/journal.pgen.1004856

Figure 7. BAN regulators are direct targets of STK.

Figure 7

(A) qRT-PCR performed on cDNA obtained from siliques from 3 to 4 DAP and from unpollinated flowers for ABS. Relative mRNA levels indicate that the expression of all the genes is up-regulated in the absence of the STK protein at 3–4 DAP; these differences are statistically significant as determined by Statistical Student's t test (P<0.01). The expression level of ABS is not affected in unpollinated flowers in the absence of the STK protein. Error bars represent the propagated error value using three replicates. (B) Schematic representation of CArG box positions. Schematic diagrams of EGL3, ABS and TT8 loci indicating the regions analysed by the ChIP experiment (black bars). Black boxes: exons; white boxes: promoters, introns, 3′ and 5′ UTRs. Asterisks indicate CArG boxes. (C) ChIP enrichment tests by qRT-PCR show that STK binds to the selected regions of ABS and EGL3. Fold enrichment was calculated over the negative controls. Error bars represent the propagated error value using three replicates.