Figure 5. MOBF12/IncF plasmid analysis.
Protein cluster analysis was performed with kClust software (parameters: 30% identity, 50% coverage) on the set of 14 plasmids shown in Table 4. Plasmid pGUE-NDM [119] was excluded from this comparison since it is only distantly related to the others (see dendrogram in Fig. 5). A total of 354 protein clusters were obtained and annotated versus the NCBI protein database (Blastp). Manual inspection was carried out to classify the reference proteins of each cluster into one of these three groups (comparative analysis shown with BRIG): (i) Backbone and metabolic proteins (panel A); (ii) Virulence and Antibiotic resistance proteins (panel B); and (iii) ISs and hypothetical proteins (not shown).