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. 2014 Dec 18;10(12):e1004894. doi: 10.1371/journal.pgen.1004894

Figure 1. Haloperidol induces pH dependent sensitivity and other biological effects in yeast.

Figure 1

(A) Growth at different pH values and haloperidol concentrations. pH 4.3: YPD at pH value 4.3; pH 7.0: YPD at pH value 7.0. Saturated cultures in liquid YPD were serially diluted 1∶10 before pinning onto agar plates. Plates were incubated at 30°C for 24–48 hr. (B) Haloperidol causes vacuole acidification related impairment. Treated versus untreated cells were stained (see Methods) with FM4-64 (stains vacuole membrane), carboxy-DCFDA (diffuses into vacuole) after 2 hours of haloperidol exposure, and quinacrine (accumulates in acidic compartments) after 6 hours of treatment with haloperidol at 150 µM.