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. 2014 Dec 18;10(12):e1004888. doi: 10.1371/journal.pgen.1004888

Figure 3. Notch signaling regulates levels of E-cadherin between GSCs and the niche.

Figure 3

(A) Mosaic germaria (one-week (w)-old control and N55e11 mutant germaria) with GFP (green, wt cells), E-cadherin (E-cad) (red), 1B1 (blue, GSC fusomes), and LamC (blue, cap cell nuclear envelopes) labels. Wt GSCs are outlined by solid lines; control or mutant GSCs (GFP-negative (−)) are outlined by dashed lines. Scale bar, 5 µm. A′, contact area between niche cap cells (green) and wt (red) and GFP− GSCs (blue). Scale bar, 1 µm. (B and C) Average fold changes (FC) of E-cad expression in GFP− GSCs, relative to those of a wt GSC within the same niche of ctrl, N55e11 (B), or NAXE-2 (C) mosaic germaria at 1, 2 or 3w after clone induction. (D and E) Contact area between niche cap cells and GFP+ GSCs or GFP− GSCs in ctrl, N55e11 (D), or NAXE-2 (E) mosaic germaria at 1, 2 or 3w after clone induction. The number of mosaic germaria analyzed is shown above each bracket. *, P<0.05; **, P<0.01; ***, P<0.001. Error bar, mean ± SEM. The genotype of the control germaria is hs-flpneoFRT19A/ubiGFPFRT19A.