Figure 4. SHF-specific loss of Vangl2 results in outflow tract defects.

A,B,E,F) Targeted deletion of Vangl2 by Wnt1-Cre, in NCC, does not result in neural tube (A,E) or outflow tract defects (B,F). C,D,G,H) In contrast, although there are no neural tube defects when Vangl2 is deleted in the Isl1-Cre expressing SHF (G), the resultant embryos do have double outlet right ventricle (H – compare with D). I–P) No defects were seen when Vangl2 was deleted in either Nkx2.5-Cre expressing cardiac progenitors or Mlc2v-Cre expressing differentiated cardiomyocytes. In each case the arrows show the communication between the ventricle and the aorta. All embryos are E14.5. Also see S4 Fig. Ao – aorta, LV - left ventricle, RV - right ventricle, Vangl2^f – Vangl2^flox. Scale bar  = 2 mm (white), 500 µm (black).