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. 2014 Dec 18;8(12):e3342. doi: 10.1371/journal.pntd.0003342

Figure 1. Demonstration of analytical specificity of pap 31 LAMP.

Figure 1

A. Lane 1: 100 bp ladder, Lane 2–7: products from LAMP reactions containing genomic DNA from R. conorii, R. rickettsii, R. typhi, Orientia tsutsugamushi, L. interrogans, and B. henselae, respectively. Lane 8: negative control, Lane 9: positive control. B. LAMP assay performed using a combination of B. bacilliformis genomic DNA (10 copies/reaction) and other bacterial genomic DNA(100 copies/reaction). Lane 1: 100 bp ladder, Lane 2–6, 9: B. bacilliformis plus either R. conorii, or R. rickettsii, or R. typhi, or Orientia tsutsugamushi, or L. interrogans, or B. henselae, respectively. Lane 7: positive control, Lane 8: negative control.