Table 1. Description of LAMP and qPCR primers used for detection of B. bacilliformis.
Primer name | Length (bp) | Tm (°C) | Sequences 5′- 3′ |
LAMP primers#2 | |||
-F3 | 22 | 58.9 | GTTTGGGTTGATAAGGAAGGTA |
-B3 | 21 | 58.7 | TGAACCAATAGCTTGTACCTG |
-FIP | 38 | 75.5 | GGCCCTTAGCGACCTCAATCCAGGGCAGAGACTCAAGA |
-BIP | 40 | 72.9 | AAAGTGGGCTGGTGCCACACATAAGGCATAATACGGTCAG |
-LF | 21 | 76.8 | AATCTGCTTGAAAGCATCTGC |
-LB | 19 | 71.8 | GTACGCATCGGTTTTGGTG |
qPCR primers#1 | |||
-Forward | 20 | 59.5 | AGGTGGTTTGTACGCAGGTT |
-Reverse | 20 | 59.1 | TCTTGAGTCTCTGCCCTGTG |