Table 2. Detection limit of the LAMP assay compared to qPCR targeting different types of known positive samples for B. bacilliformis.
Type of samples | qPCR, copies/µL@ (range) | Mean Ct value | LAMP, copies/µL |
Plasmid | 2 | 39.1 | 2 |
Genomic DNA | 3.6 (1, 7) | 38.8 | 1 |
Genomic DNA in presence of human DNA | 10 (8, 12) | 39.4 | 10 |
Genomic DNA in presence of sand fly DNA | 18 (13, 23) | 38.6 | 8 |
Mean copies number.