Table 4. Sensitivity and specificity of pap31 LAMP assay in detecting B. bacilliformis from experimentally infected sand flies compared to qPCR.
D1 | D3 | D5 | D7 | D9 | |
Sensitivity* | 100% (13/13) | 100% (7/7) | NA | NA | NA |
Specificity** | NA | 75% (3/4) | 66.6% (4/6) | 100% (7/7) | 100% (8/8) |
* Sensitivity was calculated as (number of true positive)/(number of true positives + number of false negatives)
**Specificity was calculated as (number of true negatives)/(number of true negatives+ number of false positives).