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. 2014 Nov 10;592(Pt 24):5373–5390. doi: 10.1113/jphysiol.2014.283218

Figure 1. Expression of GIRK1/2 increases the surface levels of Gβγ-YFP but not of myr-YFP-Gαi3.

Figure 1

A, examples of images of oocytes expressing fluorescently labelled proteins. Surface expression levels of myr-YFP-Gαi3 (5 ng RNA per oocyte) and Gβγ-YFP (1 and 0.5 ng RNA of Gβ and YFP-Gγ, respectively) were measured without coexpression of GIRK (left column), or with coexpression of GIRK1/2 (1 or 2 ng RNA per oocyte; right column). B, summary of experiments shown in A. GIRK1/2 increased the expression of YFP-Gβγ (dark grey bars), but not of myr-YFP-Gαi3 (light grey bars). C, coexpressed Gβγ increases the surface level of myr-YFP-Gαi3, whereas GIRK1/2 and GIRK2HA are without effect. D, at high expression level, Gβγ is not recruited to the PM by GIRK1/2. The plot shows normalized expression level of Gβγ in oocytes injected with 5 ng Gβ RNA and 1 ng Gγ RNA, with or without GIRK1/2 channel (2 ng RNA per oocyte). At this expression level, there is no detectable Gβγ recruitment. ***P < 0.001; n.s., not statistically significant (P > 0.05).