Table 1.

Nested PCR primer sequences

Gene	External/Internal	Forward (5′→3′)	Reverse (5′→3′)	Size (bp)	Tm (°C)
Cav1.1	External	CTTCCAGGTGTGAGGCCTAT	AGCTGGTTGGATGGAGTGAC	309	55
	Internal	CCAGCTGCATGGACTGAGTA	CCTGCTTCTTGCTGGTAAGG	102	55
Cav1.2	External	GCAGTATGGGAAACCCAAGA	AAAAAGCCCTACAACCACGA	373	54
	Internal	TCTGCTCTGCCTGACTCTGA	CACACAATTGGCAAAAATCG	109	54
Cav1.3	External	CATGTCCAAAGGCCTTCAAT	AGCTGCTCGTCTCCTGACTC	323	54
	Internal	TCCAGCAGGAAATTCGGTGTGTCA	TCGGTCGTGCTTGTAGGAGTAATG	187	67
Cav1.4	External	GTATCATGGCCTACGGTGGT	CCACCTTCTCCTTGGGTACA	366	57
	Internal	GCCATTGCTGTGGATAACCT	CTCCACCAGGCACCAATACT	118	56
Cav3.1	External	AACGTCCTGCTGCTCTGTTT	AGGAGTGAGCGTCCATTACG	469	55
	Internal	GCAACACCACCTGTGTCAAC	GATCCAGGCATAGCCAATGT	110	56
Cav3.2	External	AGCATCTGACTGTCCCCAAC	AACAACCTCAATGCCAAAGC	325	55
	Internal	CTGCCCAGAGAAGGAACAAG	CAGGCTCATCTCCACTGTCA	110	57
Cav3.3	External	CATCCGTATCATGCGTGTTC	GTGAGCACGAAGCTCACAAA	399	55
	Internal	CTGGTCTGCAATGACGAGAA	CCATTCCAGTTATCGCCTGT	116	55

The table shows forward and reverse primer sequences, size of the target gene and annealing temperature (T_m) of external and internal primers for rat L- and T-type Ca²⁺ channel isoforms.