Table 1.
Gene | External/Internal | Forward (5′→3′) | Reverse (5′→3′) | Size (bp) | Tm (°C) |
---|---|---|---|---|---|
Cav1.1 | External | CTTCCAGGTGTGAGGCCTAT | AGCTGGTTGGATGGAGTGAC | 309 | 55 |
Internal | CCAGCTGCATGGACTGAGTA | CCTGCTTCTTGCTGGTAAGG | 102 | 55 | |
Cav1.2 | External | GCAGTATGGGAAACCCAAGA | AAAAAGCCCTACAACCACGA | 373 | 54 |
Internal | TCTGCTCTGCCTGACTCTGA | CACACAATTGGCAAAAATCG | 109 | 54 | |
Cav1.3 | External | CATGTCCAAAGGCCTTCAAT | AGCTGCTCGTCTCCTGACTC | 323 | 54 |
Internal | TCCAGCAGGAAATTCGGTGTGTCA | TCGGTCGTGCTTGTAGGAGTAATG | 187 | 67 | |
Cav1.4 | External | GTATCATGGCCTACGGTGGT | CCACCTTCTCCTTGGGTACA | 366 | 57 |
Internal | GCCATTGCTGTGGATAACCT | CTCCACCAGGCACCAATACT | 118 | 56 | |
Cav3.1 | External | AACGTCCTGCTGCTCTGTTT | AGGAGTGAGCGTCCATTACG | 469 | 55 |
Internal | GCAACACCACCTGTGTCAAC | GATCCAGGCATAGCCAATGT | 110 | 56 | |
Cav3.2 | External | AGCATCTGACTGTCCCCAAC | AACAACCTCAATGCCAAAGC | 325 | 55 |
Internal | CTGCCCAGAGAAGGAACAAG | CAGGCTCATCTCCACTGTCA | 110 | 57 | |
Cav3.3 | External | CATCCGTATCATGCGTGTTC | GTGAGCACGAAGCTCACAAA | 399 | 55 |
Internal | CTGGTCTGCAATGACGAGAA | CCATTCCAGTTATCGCCTGT | 116 | 55 |
The table shows forward and reverse primer sequences, size of the target gene and annealing temperature (Tm) of external and internal primers for rat L- and T-type Ca2+ channel isoforms.