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AMPK and eNOS were involved in cardioprotection of HPC against H/R injury. Cell apoptosis assay measured by flow cytometry method (FCM) showing H9c2 cells apoptosis. HPC increased cell survival and decreased cell apoptosis against H/R injury, which was abolished by the AMPK inhibitor Compound C and eNOS inhibitor L-NIO, and mimicked by the AMPK activator metformin. *P < 0.05 vs. Control; #P < 0.05 vs. H/R; &P < 0.05 vs. HPC.
