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Expression of p-AMPK and AMPK in H9c2 cells. HPC and the AMPK activator metformin significantly increased the expression of p-AMPK compared with H/R group. A: Representative immunoblots of phosphorylated AMPK at residue threonine 172 (p-AMPKThr172) and total AMPK at 120 min of reoxygenation. B: Densitometric analysis of the phosphorylated state of AMPKThr172. Bars represent the ratio of phosphorylated AMPK to total AMPK. #P < 0.05 vs. H/R.
