Skip to main content
. 2014 Oct 15;7(11):7378–7388.

Figure 5.

Figure 5

Expression of eNOS and p-eNOS in H9c2 cells. HPC increased eNOSSer1177 phosphorylation compared with H/R group in H9c2 cells, which was abolished by the AMPK inhibitor Compound C and mimicked by the AMPK activator metformin. HPC did not alter eNOSThr495 phosphorylation. A: Representative immunoblots of phosphorylated eNOS at serine residue 1177 (p-eNOSSer1177), theronine residue 495 (p-eNOSThr495), and total eNOS at 120 min of reoxygenation. B: Densitometric analysis of the phosphorylated state of eNOSSer1177. C: Densitometric analysis of the phosphorylated state of eNOSThr495. #P < 0.05 vs. H/R; &P < 0.05 vs. HPC.