Table 2.

Thermal Cycling programs for semiquantitative RT-PCR of genes studied

	Glutathione peroxidase	Cu/Zn-SOD	Mn-SOD	Catalase	GAPDH (internal control, House keeping gene)
Initial denaturation	95°C for 10 minutes	95°C for 10 minutes	95°C for 10 minutes	95°C for 10 minutes	94°C for 5 minutes
Cycles
Number	27	27	26	27	25
Denaturation	94°C for 1 minute	94°C for 30 seconds	94°C for 1 minute	94°C for 1 minute	95°C for 30 seconds
primer annealing	59°C for 1 minute	56°C for 30 seconds	62°C for 1 minute	62°C for 1 minute	62.5°C for 30 seconds
Extension	72°C for 1 minute	72°C for 1 minute	72°C for 1 minute	72°C for 1 minute	72°C for 2 minute
Final extension	72°C for 10 minutes	72°C for 10 minutes	72°C for 10 minutes	72°C for 10 minutes	72°C for 7 minutes
Product size	301 bp	333 bp	410 bp	173 bp	721 bp