Figure 5. The CRA2 gene encodes a Leucine-Rich Repeat Receptor-Like Kinase (LRR-RLK).

A, Structure of the CRA2 protein indicating the 10 mutant alleles (arrowheads) that were identified by forward and reverse genetic screens (the indicated position is related to the predicted ATG) and functional domains. The vertical black bars indicate the predicted transmembrane domains; in grey are the Leucine-Rich Repeats; and the hatched region represents the kinase domain. The black arrowheads represent alleles that are linked to a Tnt1 retro-element insertion; the grey arrowheads represent another insertional element; and the white arrowhead represents a nucleotide deletion causing a translational frameshift. Bar  = 50 residues. B, Phylogenetic tree of selected LRR-RLKs that are related to CRA2 from Arabidopsis (subfamily XI) or are functionally characterized in legumes. The sequences were aligned using Muscle, and the regions that were conserved between all of the sequences were defined with Gblocks. The phylogenetic relationships were determined using a maximum likelihood analysis (PhyML), and statistical support for each node was estimated by approximate likelihood ratio tests. The Chlamydomonas reinhardtii XP001698687 protein was used to root the tree.