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. 2014 Dec 18;9(12):e110752. doi: 10.1371/journal.pone.0110752

Figure 1. BMP signaling enhances Isl1-CPC cardiac differentiation.

Figure 1

Freshly sorted EB day 5.5 GFP+ Isl1-CPC were treated with control (A, D) or 25 ng/ml Bmp4 (B, E) for 6 days, followed by immunostaining with antibodies against cardiomyocyte-specific protein cTnT (red in A and B) or labelled with cTnT and Alexa fluor 647 for FACS analysis (D, E). Nuclei: blue (Hoechst). (C) Quantification of A and B (over 2000 cells counted from 3 independent experiments). (F) Quantification of D and E (20000 cells FACS analyzed for each of 3 independent experiments). Scale bar: 100 µm; *statistical significance p<0.05, Bmp4 –treated cultures vs. control.