Figure 3. Dose response curves for amphotericin B and miltefosine against intracellular L. donovani amastigotes.

Parasites were released by conditional lysis with saponin from bone marrow derived macrophages of BALB/c mice after 72 h incubation with anti-leishmanial standard drugs. Using this method, the measured activities of amphotericin B (EC₅₀ 29 nM) and miltefosine EC₅₀ (1.45 µM) were reproducible and comparable to reported values against intracellular L. donovani (43–45). Representative plots for amphotericin B (n = 4) and miltefosine (n = 3) are shown. (R² _AmphoB – 0.9905, R² _Mil – 0.9963).