Table 1. Activity of gold standard drugs after 3-day treatment against intra- and extracellular L. donovani amastigotes.

	EC50 ± SD intracellular L. donovani amastigotes	EC50 ± SD extracellular L. donovani amastigotes*
Amphotericin B	30±9 nM	50±11 nM
Miltefosine	1.38±0.06 µM	7.85±0.5 µM

*Assay was set up with amastigotes isolated from spleens of infected Rag2−/− mice with subsequent incubation at 26°C. All assays were run in triplicate with biological repeats (intracellular amphotericin B, n = 4; intracellular miltefosine, n = 3; extracellular amphotericin B, n = 3; extracellular miltefosine, n = 2).