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. 2014 Sep 2;9(3):708–720. doi: 10.1038/ismej.2014.158

Figure 4.

Figure 4

Confocal micrographs of FISH-stained Nitrotoga-like bacteria with corresponding MAR signal. Shown are representative images from the incubation of activated sludge from WWTP Bad Zwischenahn with 0.5 mM NO2− at 14 °C. (a) Nitrotoga cell aggregates hybridized to probes Ntoga122 (red), FGall221b (green) and EUB338mix (blue); (b) corresponding DIC image showing silver grain deposition above radioactively labeled bacteria; (c) overlay of FISH and MAR signal. The scale bar in all images=25 μm.