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. 2014 Dec 18;9(12):e115252. doi: 10.1371/journal.pone.0115252

Table 3. Determination of binding of SU/anti-V3 antibodies by ELISA, in the presence or absence of heparin.

Antibodies PPR PPRcr C36 34TF10
- + - + - + - +
SU1-5 100.0 108.4 2.4 −0.4 5.9 6.9 1.3 −4.4
SU1-7 100.0 98.9 91.0 85.7 34.7 33.9 80.9 67.8
SU1-10 100.0 94.4 1.2 0.0 5.7 6.4 2.2 −0.5
SU1-30 100.0 98.0 94.1 79.7 65.2 66.6 80.5 68.7
SU2-4 100.0 100.5 96.1 90.8 88.0 84.0 94.6 83.2
SU2-5 100.0 96.9 96.9 85.6 84.7 83.2 92.4 82.1
SU2-10 100.0 100.1 76.9 64.7 64.9 57.5 41.6 31.6

Binding of FIV SU-Fc to anti-V3 antibodies detected by ELISA as described in “Materials and Methods”. Anti-V3 mAbs were incubated with SU-Fc for 1 h in the presence or absence of heparin at room temperature. Values for other SUs-Fc are percentages of the mean optical density value of PPR SU-Fc, which is regarded as 100%. Results represent one of three independent experiments. Values in bold represent loss of specific antibody reactivity to a given SU-Fc.