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. 2014 Dec 18;9(12):e115357. doi: 10.1371/journal.pone.0115357

Figure 4. Curvature preference of the Atg8 conjugation reaction.

Figure 4

(A) Conjugation of Atg8 to individual liposomes of varying size as measured by the SLiC-assay [25]. The Atg8-PE density, Inline graphic, is plotted as a function of the vesicle radius, Inline graphic, after 20 min incubation with 125 nM Atg8 for n = 366 vesicles. The insets show snapshots of immobilized liposomes. (B) Atg8-PE density (same data) as a function of membrane curvature, Inline graphic.