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MCF10A cells were treated with 4OHT for 24 hours to stimulate transgene expression. Cells were then either treated with vehicle (DMSO) or MG132 for 4 hours prior to harvest and immunoprecipitation for MYC. Immunoblotting was performed for SUMO1 and SUMO2/3 revealing that SUMOylation of MYC was enhanced following proteasomal inhibition. This effect was independent of the amount of MYC present in the pulldown (lower panel).
