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. 2014 Dec 18;10(12):e1004578. doi: 10.1371/journal.ppat.1004578

Figure 3. For drp2b, increased flg22-induced ROS production is sensitive to Wortmannin.

Figure 3

(A) After a one-hour pretreatment in the absence (square) or presence (triangle) of 30 µM Wortmannin (Wm), ROS production was measured over 40 minutes in drp2b-2 (2b-2, closed symbols) and Col-0 (open symbols) in response to 1 µM flg22 (n = 24/treatment and genotype). (B) Total ROS production based on time-course experiments shown in (A) indicates that in drp2b-2 (2b-2, black bars), the increase in flg22-induced ROS production is Wm-sensitive (compare 2b-2+Wm vs Col-0+Wm, P>0.5). Experiments were done using five-to-six week old leaf tissue and repeated at least three independent times with similar results. Values are mean ± SE. Statistical analysis was done as in Fig. 1. Relative Light Units, RLU.