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. 2014 Dec 18;10(12):e1004578. doi: 10.1371/journal.ppat.1004578

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© 2014 Smith et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) and (D) Time-course of ROS production in Col-0 (open symbol) and drp2b-2 (2b-2; filled symbol) in response to living (A) Pto hrcC^- or (D) Pto DC3000 (OD₆₀₀ = 0.1). (n = 32/genotype). (B) and (E) Compared to Col-0, total ROS production was significantly increased in drp2b-2 (2b-2) after elicitation with (B) Pto hrcC⁻ (P<0.01) or (E) Pto DC3000 (P<0.007) based on values shown in (A) or (D), respectively. Relative Light Units, RLU. (C) and (F) Using qRT-PCR, PR1 mRNA levels were significantly reduced in drp2b-2 (black bars) compared to Col-0 (white bars) at 24 hr after infiltration with (C) Pto hrcC⁻ (OD₆₀₀ = 0.02) (P<0.045) or (F) Pto DC3000 (DC; OD₆₀₀ = 0.02) (P<0.005). Infiltration with water (white bars) served as mock control. (n = 6/genotype and treatment). All experiments were done using five-to-six week old leaf tissue and repeated at least three independent times with similar results. Values are mean ± SE. Statistical analysis was done as in Fig. 1.