Figure 4. GLUT1^sel cells can be induced to express pericytic/SMC phenotype and undergo adipogenesis.

(A): Schematic of pericyte/SMC differentiation assay: 5 days of co-culture with ECFCs, followed by removal with anti-CD31-coated magnetic beads. (B): CD31 and αSMA expression measured by flow cytometry of saponin-permeabilized cells. (C): Calpoinin I and sm22α measured by western blot. CD31 shown to evaluate ECFC removal. ACTB used as the loading control. (D): QPCR to analyze mRNA levels of pericyte/SMC markers. N=4, *P<0.05 and **P<0.01 by ANOVA followed by paired t test. (E): Cells induced to undergo adipogenesis stained with Oil Red-O. HemSC and mesenchymal stem cells served as positive controls; ECFC served as negative control. Scale bar, 80 μm. Results are representative of independent assays using paired GLUT1^sel and GLUT1^negCD31⁺ cells isolated from 4 different IH specimens.