Figure 2. 1H-15N-HSQC spectra of SR-BI(405–475) in different detergent micelles.
Purified [U-15N]-SR-BI(405–475) was solubilized in water containing 5% detergent, 10% D2O, and 0.02% sodium azide at pH 6.8 and screened by NMR analyses. The following detergents were tested: (A) OG; (B) DDM; (C) DPC; (D) LMPG; (E) LPPG; and (F) LOPG. Anticipated side chain cross peaks are boxed.