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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: FEMS Yeast Res. 2014 Oct 10;14(8):1171–1185. doi: 10.1111/1567-1364.12210

Figure 2. Use of directed pop-out YIPs to enable single locus integration with transformation-free selection marker rescue.

Figure 2

Representative flow cytometry histograms for strains integrated with a GFP expression construct into different loci before and after selection marker pop-out. The marker used to integrate GFP into the fcy1, leu2, and lys5 loci was KlURA3; the engineered counterselectable marker FCY1-T2A-SpHIS5 was used to integrate GFP into the his3 locus.