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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: FEMS Yeast Res. 2014 Oct 10;14(8):1171–1185. doi: 10.1111/1567-1364.12210

Figure 3. Design and validation of a set of new counterselectable markers.

Figure 3

Each of the counterselectable markers links positive selection with the negatively selectable 5-FCS phenotype.. Images show wild-type (CEN.PK2) and fcy1 (fcy1::KlURA3) controls and fcy1 strains transformed with leu2-targeting YIPs carrying Fcy1p-T2A-fusion versions of the four positive selection markers tested: A. KanMX, B. hphNT1, C. TmTrpF, and D. SpHIS5 (3 biological replicates of each are shown).