Table 1.
Description of plasmids used in this study.
| Plasmid | Description | E.coli Selection | Reference |
|---|---|---|---|
| Previously described plasmids used in this study | |||
| pCS827 | pMB E. coli plasmid sequence | AmpR | Hawkins & Smolke (2008) |
| pMK-RQ | pMK E. coli plasmid sequence | KanR | GeneArt/Life Tech |
| pUG6 | LoxP-KanMX | AmpR | Gueldener et al. (2002) |
| pUG27 | LoxP-SpHIS5 | AmpR | Gueldener et al. (2002) |
| pUG72 | LoxP-KlURA3 | AmpR | Gueldener et al. (2002) |
| pSA40 | LoxP-CaLYS5 | AmpR | Ito-Harashima & McCusker (2004) |
| pRS41H | hphNT1 | AmpR | Taxis & Knopp (2006) |
| pAG303GPD-ccdb | Transient YIP targeting HIS3 | AmpR | Alberti et al. (2007) |
| pAG305GPD-ccdb | Transient YIP targeting LEU2 | AmpR | Alberti et al. (2007) |
|
| |||
| Insert plasmids containing fluorescent reporter genes | |||
| pCS2952 | [tag3]P_GPD-GFP-T_ADH1-[tag4] | KanR | This study |
| pCS2953 | [tag3]P_GPD-mCherry-T_Cyc1-[tag4] | KanR | This study |
| pCS2954 | [tag3]P_GPD-BFP-T_ADH1-[tag4] | KanR | This study |
|
| |||
| Insert plasmids containing yeast selection markers for assembling new directed pop-out YIPs | |||
| pCS2921 | [tag1]-loxP-KanMX-[tag3] | KanR | This study |
| pCS2932 | [tag1]-loxP-FCY1-T2A-KanMX-[tag2] | KanR | This study |
| pCS2933 | [tag1]-loxP-FCY1-T2A-hphNT1_MX-[tag2] | KanR | This study |
| pCS2934 | [tag1]-loxP-FCY1-T2A-TmTrpF_MX-[tag2] | KanR | This study |
| pCS2935 | [tag1]-loxP-FCY1-T2A-SpHIS5_MX-[tag2] | KanR | This study |
| pCS2936 | [tag1]-loxP-CaLYS5-[tag2] | KanR | This study |
| pCS2937 | [tag1]-KlURA3-[tag2] | KanR | This study |
|
| |||
| YIPin plasmids for assembling YIPs targeting loci demonstrated in this study | |||
| pCS2940 | FCY1::[tag1]-RFP-[tag4] | AmpR | This study |
| pCS2941 | HIS3::[tag1]-RFP-[tag4] | AmpR | This study |
| pCS2942 | LEU2::[tag1]-RFP-[tag4] | AmpR | This study |
| pCS2943 | LYS5::[tag1]-RFP-[tag4] | AmpR | This study |
|
| |||
| YIPs for testing new counterselectable markers alone in LEU2 locus | |||
| pCS2947 | LEU2::[tag1]-FCY1-T2A-KanMX-[tag4] | AmpR | This study |
| pCS2948 | LEU2::[tag1]-FCY1-T2A-hphNT1-[tag4] | AmpR | This study |
| pCS2949 | LEU2::[tag1]-FCY1-T2A-TmTrpF-[tag4] | AmpR | This study |
| pCS2950 | LEU2::[tag1]-FCY1-T2A-SpHIS5-[tag4] | AmpR | This study |
| pCS2951 | LEU2::[tag1]-CaLYS5-[tag4] | AmpR | This study |
|
| |||
| YIP for generating fcy1 phenotype | |||
| pCS2965 | FCY1::[tag1]-KanMX-[tag4] | AmpR | This study |
|
| |||
| Directed pop-out YIPs for integration of fluorescence reporter | |||
| pCS2956 | FCY1::Kl_URA3-GFP | AmpR | This study |
| pCS2957 | HIS3::Kl_URA3-GFP | AmpR | This study |
| pCS2958 | LEU2::Kl_URA3-GFP | AmpR | This study |
| pCS2959 | LYS5::Kl_URA3-GFP | AmpR | This study |
| pCS2963 | HIS3::FC-TmTrpF-mCherry | AmpR | This study |
| pCS2964 | LYS5::FC-SpHIS5-BFP | AmpR | This study |
| pCS2966 | HIS3::FC-SpHIS5-GFP | AmpR | This study |