Skip to main content
. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2014 Nov 6;35(1):175–183. doi: 10.1161/ATVBAHA.114.304786

Fig. 2. SMC-specific PTEN deletion promotes ex vivo aortic calcification.

Fig. 2

A) SMC-specific PTEN deficiency increased phosphorylated AKT in the media. Consecutive sections from descending aortas from PTENf/f and PTENΔ/Δ mice were stained with H&E (histology), VVG (elastin) or specific antibodies for PTEN, SMA, and phosphorylated AKT (pAKT). Representative images from 5 pairs of littermates are shown. B) SMC-specific PTEN deficiency promoted aortic calcification. Aorta rings from PTENf/f and PTENΔ/Δ mice were cultured in osteogenic medium containing H2O2 (0.3mM) for 2 weeks. Consecutive sections were stained by H&E, Alizarin Red (calcium), Von Kossa (calcium phosphate), and specific antibodies for pAKT and Runx2. Representative images from 5 independent experiments are shown.