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. 2014 Sep 19;132(1):85–98. doi: 10.1111/jnc.12934

Figure 2.

Figure 2

Epidermal fatty acid binding protein (E-FABP) is up-regulated under palmitic acid-induced lipotoxicity (PAM-LTx) in NGFDPC12 cells. (a) Cells were treated with control medium (CTL) or PAM at the concentrations of 75, 150, or 300 μM for 6–48 h. Quantification of E-FABP mRNA levels was performed by real-time RT-PCR. (b) Cells were treated with CTL or 300 μM PAM for 6–24 h. Quantification of E-FABP protein levels was done by Western blots followed by densitometry analysis, using β-actin as the loading control. (c) A representative Western blot for E-FABP (15 kDa) and β-actin (42 kDa) of NGFDPC12 cells treated with 300 or 150 μM PAM for 6–24 h. The data represent mean ± SEM of three independent experiments. Significance symbols: **p < 0.01 and ***p < 0.001 (compared to control group).