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. 2014 Sep 19;132(1):85–98. doi: 10.1111/jnc.12934

Figure 6.

Figure 6

Lowering epidermal fatty acid-binding protein (E-FABP) levels in NGFDPC12 cells by siRNA increases reactive oxygen species (ROS) accumulation and PAM-LTx-induced cell death. (a) Left: Three-day-differentiated PC12 cells were transfected with siControl or siE-FABP for 5 days. A representative E-FABP Western blot is shown. Right: siControl- and siE-FABP-transfected cells were treated with control medium or 300 μM PAM for 12 and 18 h. Relative E-FABP mRNA levels were determined by real-time RT-PCR. (b) Cellular ROS levels of transfection reagent (vehicle), siControl and siE-FABP treated cells were measured by H2DCFDA method at 12 and 18 h after PAM (or control medium) treatment. (c) Cell viability of siControl and siE-FABP cells were measured by WST-1 assay at 24 and 48 h after PAM treatment. CTL: control medium at 48 h. The data represent mean ± SEM of three independent experiments. Significance symbols: *p < 0.05, **p < 0.01 and ***p < 0.001. Significance symbols are shown above bars when compared to control group and are shown above lines when compared between two linked groups.