Skip to main content
. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Placenta. 2014 Oct 5;35(12):1013–1020. doi: 10.1016/j.placenta.2014.09.015

FIG 2. Placental gene expression changes with maternal obesity.

FIG 2

Gene expression in the placental compartments junctional zone, labyrinth zone and metrial gland, and fetal liver were assessed using RNA-seq. Reads Per Kilobase per Million mapped reads (RPKM) values for all Refseq genes were calculated using Avadis-NGS. (A) Principal Component Analysis of all genes showing expression profiles of individual samples belonging to the same compartment are highly related and group together. (B) Hierarchical clustering the union of genes altered due to maternal obesity in each zone are presented. Differentially expressed genes between lean and OB groups were identified based on P value ≤ 0.05 using Audic-Claverie test and ±1.4- fold change. A complete list is included in supplementary material. Heat-map colors red, white, and blue represent up-regulation, no relative effect, and down-regulation of transcripts, respectively. (C) Venn diagram of genes altered with maternal obesity in each zone. Enrichment of GO biological processes terms within differentially expressed genes in (D) metrial gland (E) junctional zone (F) labyrinth zone and (G) fetal liver. Gene ontology enrichment was carried out using GoRilla.