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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Circ Cardiovasc Genet. 2014 Jul 21;7(5):615–624. doi: 10.1161/CIRCGENETICS.113.000398

Figure 4.

Figure 4

Assessment of platelet activation by flow cytometry. Platelet rich plasma from TLR4loxP/Pf4-cre and TLR4loxP/loxP mice was isolated and stained for CD41 and CD62p (100uM ADP). A: Percentage of CD62p positive. Data are means ± SEM, n = 8–10 mice/group. *p<0.05 vs. control. B: Representative dual parameter dot-plot figures obtained by flow cytometry for CD41 and CD62p. C: Treatment of PRP from both TLR4loxP/Pf4-cre and TLR4loxP/loxP mice with 0.1U/ml thrombin confirms equal CD62p upregulation. Data are means ± SEM, n = 5 mice/group. p=NS between thrombin groups.