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. Author manuscript; available in PMC: 2015 Jun 18.
Published in final edited form as: Nature. 2014 Oct 22;516(7531):423–427. doi: 10.1038/nature13902

Extended Data Figure 4. Induction of the Eml4-Alk inversion in primary MEFs using an adenoviral vector expressing FLAG-Cas and tandem sgRNAs.

Extended Data Figure 4

(a) Schematic of the Adenoviral vectors. (b) Immunoblot using a anti-FLAG antibody on lysates from MEFs infected with the indicated adenoviruses. (c) Small-RNA northerns using probes against sgEml4 and sgAlk on total RNAs from cells infected with Ad-Cas9 or Ad-EA. (d) PCR-mediated detection of the Eml4-Alk inversion in MEFs infected with Ad-Cas9 or Ad-EA for the indicated number of days. (e) Standard curve generated performing quantitative PCR analysis on genomic DNA containing known fraction of Eml4-Alk alleles. Average of two independent experiments. (f) Quantification of the fraction of MEFs harboring the Eml4-Alk inversion at the indicated time points after infection with Ad-EA or Ad-Cas9. Values are mean of three independent infections ± s.d.