Extended Data Table 4.
Primer pairs and PCR reactions.
| Name | Description | Expected size (bp) |
|---|---|---|
| Alk_gDNA-for | Surveyor assay | 961 |
| Alk_gDNA-rev | ||
| Eml4_gDNA-for | Surveyor assay | 602 |
| Eml4_gDNA-rev | ||
| Eml4_gDNA-for | Eml4-Alk genomic | 527 |
| Alk_gDNA-rev | ||
| Alk_gDNA-for | Alk-Eml4 genomic | 1036 |
| Eml4_gDNA-rev | ||
| Eml4_gDNA-for | Deletion | 1044 |
| Alk_gDNA-for | ||
| miR17-92_gDNA-for | Control (gDNA) | 255 |
| miR17-92_gDNA-rev | ||
| GAPDH_cDNA-for | Control (cDNA) | 237 |
| GAPDH_cDNA-rev | ||
| Eml4-for | Eml4-Alk genomic (three primers) |
Eml4: 240 Eml4-Alk: 190 |
| Eml4-rev | ||
| Alk-rev | ||
| Eml4_cDNA-for | Eml4-Alk transcript (three primers) |
Eml4: 336 Eml4-Alk: 276 |
| Eml4_cDNA-rev | ||
| Alk_cDNA-rev | ||
| Eml4_cDNA-for | Eml4-Alk transcript (junction) | 276 |
| Alk_cDNA-rev | ||
| Eml4_Exon1_for | Eml4-Alk transcript (full length) | 3238 |
| Alk_Exon29_rev | ||
| Npm1_gDNA-for | Npm1-Alk genomic | 581 |
| Alk_gDNA-rev | ||
| Alk_gDNA-for | Alk-Npm1 genomic | 1036 |
| Npm1_gDNA-rev | ||
| Npm1_cDNA-for | Npm1-Alk transcript | 404 |
| Alk_cDNA-rev |
This table lists the primer pairs and the sizes of the expected products for each PCR reaction described in this study.