Table 1.

Relationship in the F2 progeny of an SHR-A3 x SHR-B2 cross between inheritance of SHR-A3 marker alleles at 4 proposed renal injury loci and renal injury measures. Injury scores are presented for each of the parental lines, along with the difference between the lines (Delta A3 vs B2). We were interested to know, in F2 animals, whether a relationship existed between inheritance of SHR-A3 alleles at the 4 proposed renal injury loci and observed renal injury and how much of the parental injury Delta might be accounted for by such inheritance. The relationship between inheritance of SHR-A3 alleles and renal injury scores was observed by linear regression of scores for each injury measure and the number of SHR-A3 injury loci alleles inherited in each of 210 F2 animals. The injury measure in F2 animals that inherit from 0 of 8 or 8 of 8 SHR-A3 haplotype block marker alleles was estimated from the regression equation. This estimation did not require extrapolation beyond the actual data obtained (i.e. F2 animals included individuals inheriting as many as 8 SHR-A3 marker alleles and as few as 0). The combined effect size of these loci on injury is shown as the delta between the estimated injury score of F2 animals inheriting 0 or 8 SHR-A3 alleles. The percentage of parental strain renal injury difference that can be attributed inheritance of 0 or 8 SHR-A3 alleles in F2 animals is shown. The p values provided indicate the significance of the regressions obtained by ANOVA. uACR indicates urinary albumin to creatinine ratio, TI indicates tubulo-interstitial, Glom indicates glomerular.

	Parental lines		F2 animals
Injury Trait	SHR-A3	SHR-B2	Delta (A3 vs B2)	A3 alleles 8/8	A3 alleles 0/8	Delta (8 vs 0 alleles)	% parental difference explained	p value
uACR (mg/mg)	21.94	4.86	17.08	14.28	3.03	11.25	65.9	0.0124
Fibrosis score	3.65	2.5	1.15	3.14	2.36	0.78	67.8	0.0016
TI injury score	2.62	1.44	1.18	2.02	1.32	0.69	58.9	0.0076
Glom injury score	1.65	1.26	0.38	1.4	1.24	0.16	41.9	0.2608