Figure 2. The efficiency of HIV-based lentivirus transducing Cultured HTM cells in vitro.

Cultured HTM cells were transduced with lentivirus containing EGFP gene. Five days later, EGFP could be detected in the transfected cells (A and B are null-vector controls for C and D, respectively; A and C are brightfield images, while B and D are fluorescent images; scale bar=50 µm). The ratios of EGFP positive cells were detected by flow cytometric analysis. Health HTM cells were gated based on forward scatter (FSC) and side scatter (SSC). Representative flow cytometry plots of E and F are from those cells transduced by null-vector controls, respectively the G and H are from those cells transduced by lentivirus containing EGFP with MOI of 15. E and G show cell population of interested, while F and H show EGFP positive cell ratio. After HTM cells were transfected with HIV-based lentiviral vector containing EGFP at a different range of MOI, the ratio of EGFP positive cells was calculated based on the following formula: (EGFP positive cells/HTM total cells)×100%, as illustrated in Figure 2I. Data are shown as mean ±SD (n= 3) of three independent experiments.