FIG. 4.
Fas-induced increases in HPC and MP TF procoagulant activity involve PS. Primary HPCs from HPC TF(+) (TFflox/flox) mice or HPC TF(−) (TFflox/flox/AlbCre) mice were treated with ActD (0.2 μg/ml) or its vehicle (DMSO, 0.1%), then 30 min later with Jo2 (0.5 μg/ml) or its vehicle (PBS) for 8 h. (A) Annexin V staining of HPCs was measured using flow cytometry. (B–C) 15 min prior to factor Xa generation, cells were pre-treated with high-affinity PS-binding protein, lactadherin (5.5 μg/ml) or vehicle (PBS). TF procoagulant activity of HPCs and HPC-derived MPs was assessed by measuring factor Xa generation (see Materials and Methods). Data are represented as mean + SEM for five independent experiments. *p < 0.05 versus genotype-matched vehicle-treated HPCs (panel A).