. 2014 Apr 25;210(8):1188–1197. doi: 10.1093/infdis/jiu236

Table 1.

Primary and Nested Primer Sequences for Gametocyte Genotyping Markers

Marker	Primer	Sequences (5′->3′)
Pfs230
Primary	Pfs230_PF	AAG ACA TGT CGC CCA GGG ATA
Primary	Pfs230_PR	TTC TTC TTC ATC ACC AAA TGG ATA T
Nested	Pfs230_NF	VIC - CAG GGA TAA TTT TGT AAT RGA TGA TG^a
Nested	Pfs230_NR	ACC TTG CCT TTC TTT TTC ATC TAC A - tail
Pfg377
Primary	Pfg377_PF	CAC AAC GAA GGT TAT ATA CCT CAT AC
Primary	Pfg377_PR	TCC ATT CTT CTT TAA GGT TCG CTT C
Nested	Pfg377_NF	6FAM - GAA GAT GAC GAA GGG GAT GAA G
Nested	Pfg377_NR	CTG TAA GAA TTG GTT ATT ACT TTT GTG G - tail
PF11.1
Primary	Pf11.1_PF1^b	GAT ATA TTC TAA TAA T\|TG TTC CAA TGG
	Pf11.1_PF2	AAG TGC AGG GGA TAG TGC AG
	Pf11.1_PR	CGG TAA TAC CAT AAG CTC CTC CT
Nested	Pf11.1_NF	6FAM - GGA ATA AGG ATG ATG ATG ACG AA
Nested	Pf11.1_NR	AAC CTT CAA ATT CTT TGT CTC TTT C - tail
PF11_0214
Primary	PF11_0214_PF	TCG AGA CAA ATT GAA AAG TTA TGG
Primary	PF11_0214_PR	TTA GTG GAT AAA TGA ATA TCT ACC G
Nested	PF11_0214_NF	6FAM - AAT GAT ACA GAT TGT GAA GAA TGG T
Nested	PF11_0214_NR	TGA GGA ATA TCG TTT TGT ATA AAT GTT - tail
PFI1210w
Primary	PFI1210w_PF	TTG ATA AGG GAT ATA TAC ACA ACC ATA
Primary	PFI1210w_PR	TTC CCG TTG TGT ATT TAA GTA GAA T
Nested	PFI1210w_NF	6FAM - TGT TTC AAT TTA CCA TCT TTC TTT TC
Nested	PFI1210w_NR	GTT TTT CAA TTT TTA TGT TGT TCT CCA - tail
PFL0545w
Primary	PFL0545w_PF	GGA AGG AAA CGA AGA AGA AAC A
Primary	PFL0545w_PR	AAA GAT TGA AAT GGA GAT TCA CCT
Nested	PFL0545w_NF	VIC - TGA CAA AGG GCA CTT TAT TAT TT
Nested	PFL0545w_NR	TTT CTT CAA CAG CAT TTT GCA T – tail

^a Primer sequence contains a wobble: R = A/G.

^b Pf11.1_PF1 is spanning an intron boundary indicated by “|”.