FIG. 5.

Tissue specificity of cmlc2 promoter. The figures show DAPI-mounted 72 hpf zebrafish. In all panels, the ventricle (V) and atrium (A) are indicated by arrows. The top panel shows a wild-type embryo expressing GFP from the cmlc2 promoter. The middle panel shows an embryo injected with the cmlc2:caAHR-2AtRFP DNA, stained with anti-cyp1a antibodies shown as green. The same fish is shown in the bottom panel with the tRFP indicated by red fluorescence. Close examination shows the signal restricted to the heart in both specimens.