FIG. 9.

Mosaic expression of caAHR in cardiomyocytes and heart malformation. Embryos were injected with cmlc2:caAHR-2AtRFP as described in the Materials and Methods and collected at 96 hpf for immunostaining with antibodies against tRFP (red) and Cyp1a (green) and stained with DAPI (blue). (A) Low magnification confocal images of four representative individuals merging all three colors. Lateral views; specimens are positioned such that the heart is at center with the eye at the left edge and the yolk sac is at right. The arrow points to the heart; the merge between RFP and Cyp1a shows as yellow. Higher magnification images of the hearts are shown below each individual. (B) RFP indicating mosaic regions of caAHR expression. (C) Cyp1a immunostaining for comparison. The atrium is indicated by A and the ventricle by V. The images represent examples from sets of n = 7 for mosaic and n = 7 for whole-heart expression.